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KMID : 1161520110150030189
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Animal Cells and Systems
2011 Volume.15 No. 3 p.189 ~ p.196
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Effects of nonylphenol and 3,3′,4,4′,5-pentachlorobiphenyl on in vitro oocyte steroidogenesis in redlip mullet, Chelon haematocheilus
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Baek Hea-Ja
Hwang In-Joon
Lee Young-Don
Kim Hyung-Bae
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Abstract
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We investigated the in vitro effects of nonylphenol (NP) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) on steroidogenesis in redlip mullet, Chelon haematocheilus, oocytes. In experiment 1, we investigated the effects of NP and PCB126 on steroid production from exogenous steroid precursors. Vitellogenic oocytes (0.75 mm in diameter) were incubated with 10 and 100 ng/ml NP or PCB126 with [3H]17α-hydroxyprogesterone as a precursor. The major metabolites produced were androstenedione, testosterone (T), estrone, and estradiol-17β (E2). Both NP and PCB126 increased T production and decreased E2 production, except for 100 ng/ml PCB126. In experiment 2, oocytes (0.65?0.75 mm in diameter) were exposed to NP and PCB126 at different concentrations (0.01, 0.1, 1, 10, and 100 ng/mL). After the incubation, T and E2 production was measured by radioimmunoassay. NP inhibited E2 production at concentrations of 0.01 and 0.1 ng/ml in 0.75-mm-diameter oocytes. NP at 1 and 100 ng/mL stimulated T production, but had no observable effect on E2 production. PCB126 treatment did not affect E2 production at any of the concentrations tested. NP alone at 0.1 ng/mL resulted in a significant decrease in E2 production in 0.65-mm-diameter oocytes. PCB126 did not show any significant effects on either T or E2 production at all concentrations tested. These results suggest that NP acts like an antiestrogen at lower concentrations (0.01?0.1 ng/ml) in vitellogenic oocytes of redlip mullet.
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KEYWORD
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nonylphenol, 3,3?,4,4?,5-pentachlorobiphenyl, redlip mullet, oocytes, steroidogenesis, antiestrogens
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